Working Group on Metagenomics in Biodiversity and Ecosystems

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The Thematic Services Working Groups

The LifeWatch ERIC Thematic Services, co-developed by the LifeWatch ERIC Common Facilities and National Distributed Centres, are a key component of the 2022-2026 Infrastructure Strategic Working Plan (SWP). They represent the main priority areas of e-Service construction in LifeWatch ERIC proposed by the National Distributed Centres.

Activities, developments and physical outcomes of the LifeWatch ERIC Thematic Services, as e-Services, Virtual Labs (vLabs) and more complex and complete Virtual Research Environments (VREs), are planned to be implemented by Thematic Service Working Groups participated by scientists from both the National Distributed Centres and the Common Facilities, with an overall coordination of the LifeWatch ERIC Service Centre.

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Metagenomics in Biodiversity and Ecosystems

A brief overview

The Metagenomics in Biodiversity and Ecosystems Working Group aims to promote innovative approaches for biodiversity monitoring across terrestrial and aquatic ecosystems, supporting ecosystem health assessment, early detection of ecological changes, and sustainable resource management. By enabling rapid and cost-effective biodiversity surveys, metagenomics provides essential tools for the timely assessment of biodiversity, guiding conservation actions and strengthening long-term ecosystem resilience research.

Metagenomics has become a cornerstone technology for biodiversity monitoring. By sequencing DNA directly extracted from environmental samples (e.g., eDNA from seawater, freshwater, sediments, soil, and air), it enables the characterisation of microbial communities without culturing, providing an unprecedented and comprehensive view of species diversity, community composition, and ecosystem functioning across large and difficult-to-sample environments. In addition, eDNA recovered through shotgun metagenomic approaches can also provide signals of macrobial organisms, enabling the detection of plants, animals, and other multicellular taxa present in the ecosystem.

Metagenomics reveals both dominant and rare taxa within biological communities, enabling early detection of pollution, habitat degradation, and climate-driven biodiversity shifts. It also facilitates the discovery of novel genes and organisms, deepening our understanding of ecosystem dynamics and adaptive capacity.

Advances in high-throughput sequencing and bioinformatics have made large-scale metagenomic monitoring increasingly feasible and affordable, enabling coordinated, large-scale and long-term biodiversity monitoring programs. The WG will promote standardised methodologies, robust computational infrastructures, and accessible analysis platforms such as Galaxy instances.

Through the integration of expertise from LifeWatch ERIC, ELIXIR-IT, and the broader biodiversity research community, the Metagenomics in Biodiveristy and Ecosystems WG will contribute to building a coordinated monitoring network and developing next-generation services for biodiversity assessment, supporting both scientific research and evidence-based conservation strategies.

Join us in advancing biodiversity monitoring through cutting-edge eDNA metagenomics and bioinformatics. Together, we can build standardised, accessible, and sustainable solutions to better characterise biological communities, understand their dynamics within ecosystems, detect ecological threats at an early stage, and support the preservation of vulnerable ecosystems in a rapidly changing world.

A brief overview

The Metagenomics in Biodiversity and Ecosystems Working Group aims to promote innovative approaches for biodiversity monitoring across terrestrial and aquatic ecosystems, supporting ecosystem health assessment, early detection of ecological changes, and sustainable resource management. By enabling rapid and cost-effective biodiversity surveys, metagenomics provides essential tools for the timely assessment of biodiversity, guiding conservation actions and strengthening long-term ecosystem resilience research.

Metagenomics has become a cornerstone technology for biodiversity monitoring. By sequencing DNA directly extracted from environmental samples (e.g., eDNA from seawater, freshwater, sediments, soil, and air), it enables the characterisation of microbial communities without culturing, providing an unprecedented and comprehensive view of species diversity, community composition, and ecosystem functioning across large and difficult-to-sample environments. In addition, eDNA recovered through shotgun metagenomic approaches can also provide signals of macrobial organisms, enabling the detection of plants, animals, and other multicellular taxa present in the ecosystem.

Metagenomics reveals both dominant and rare taxa within biological communities, enabling early detection of pollution, habitat degradation, and climate-driven biodiversity shifts. It also facilitates the discovery of novel genes and organisms, deepening our understanding of ecosystem dynamics and adaptive capacity.

Advances in high-throughput sequencing and bioinformatics have made large-scale metagenomic monitoring increasingly feasible and affordable, enabling coordinated, large-scale and long-term biodiversity monitoring programs. The WG will promote standardised methodologies, robust computational infrastructures, and accessible analysis platforms such as Galaxy instances.

Through the integration of expertise from LifeWatch ERIC, ELIXIR-IT, and the broader biodiversity research community, the Metagenomics in Biodiveristy and Ecosystems WG will contribute to building a coordinated monitoring network and developing next-generation services for biodiversity assessment, supporting both scientific research and evidence-based conservation strategies.

Join us in advancing biodiversity monitoring through cutting-edge eDNA metagenomics and bioinformatics. Together, we can build standardised, accessible, and sustainable solutions to better characterise biological communities, understand their dynamics within ecosystems, detect ecological threats at an early stage, and support the preservation of vulnerable ecosystems in a rapidly changing world.

In the spotilight

Key Objectives

  1. Built a set of best practices and recommendations to optimise metagenomic experimental design according to the specific biological question and ecosystem context.
  2. Define guidelines for appropriate sampling strategies, replication, metadata collection, and sequencing approaches tailored to different monitoring objectives.
  3. Establish standard operative procedures (SOPs) and robust protocols for nucleic acid extraction from diverse environmental matrices (e.g. seawater, freshwater, sediments, biofilms, soil, air), ensuring reproducibility and high-quality downstream sequencing data.
  1. Deploy robust computational environments for large-scale analyses.
  2. Provide pre-configured Virtual Machines and Galaxy Instances for democratised access.
  3. Support workflow development for eDNA, DNA metabarcoding, and shotgun metagenomics.
  1. Foster engagement of the scientific community working on biodiversity and ecosystem monitoring, promoting the integration of molecular (NGS-based) approaches into existing research frameworks.
  2. Involve industry stakeholders and biotech/environmental companies interested in applied biodiversity studies, including environmental monitoring, bioprospecting, and
    sustainability assessment.
  3. Organise workshops, training activities, and collaborative working tables to facilitate knowledge exchange, standard adoption, and co-development of application-oriented
    services.
  4. Promote the translation of metagenomic data into actionable knowledge supporting conservation policies, environmental management, and innovation.

Photo by Alex Moliski

Coordinator

Timeline

Experimental design and extraction protocols
March–June 2026
Kick-off meeting; collection of use cases; mapping of existing experimental designs and nucleic acid extraction protocols. 
July–December 2026
Comparative evaluation of sampling strategies and sequencing approaches; assessment of extraction methods across environmental matrices; drafting of best-practice guidelines. 
January–April 2027
Pilot validation, community feedback, and release of Version 1.0 standardised guidelines. 
Bioinformatics services and infrastructure
March–September 2026
Assessment of computational needs; deployment of Virtual Machines and Galaxy Instances; integration of workflows for eDNA, DNA metabarcoding, and shotgun metagenomics. 
October 2026–June 2027
Release and optimisation of reproducible workflows; scaling of infrastructure; integration of ML/AI tools for biodiversity analyses. 
Community engagement and application integration

March–June 2026​ Stakeholder mapping and engagement strategy; first open webinar.

July 2026–December 2027​ Workshops, training activities, and industry–academia roundtables; co-development of application-oriented pilot studies.

2028​ Consolidation of collaborations, cross-infrastructure initiatives, and dissemination of impact- oriented outputs.

Experimental design and extraction protocols

  • March–June 2026
    • Kick-off meeting;
    • collection of use cases;
    • mapping of existing experimental designs and nucleic acid extraction protocols.
  • July–December 2026
    • Comparative evaluation of sampling strategies and sequencing approaches;
    • assessment of extraction methods across environmental matrices;
    • drafting of best-practice guidelines.
  • January–April 2027
    • Pilot validation, community feedback, and release of Version 1.0 standardised guidelines.

Bioinformatics services and infrastructure

  • March–September 2026
    • assessment of computational needs;
    • deployment of Virtual Machines and Galaxy Instances;
    • integration of workflows for eDNA, DNA metabarcoding, and shotgun metagenomics.
  • October 2026–June 2027
    • release and optimization of reproducible workflows;
    • scaling of infrastructure;
    • integration of ML/AI tools for biodiversity analyses.

Community engagement and application integration

  • March–June 2026
    • stakeholder mapping and engagement strategy;
    • first open webinar.
  • July 2026–December 2027
    • workshops, training activities, and industry–academia roundtables;
    • co-development of application-oriented pilot studies.
  • 2028
    • consolidation of collaborations, cross-infrastructure initiatives, and dissemination of impact- oriented outputs.

Fund raising

  • End of January 2025 – Establishing a WG Committee on scouting project application opportunities and fundraising.
Greece

The Greek National Distributed Centre is funded by the Greek General Secretariat of Research and Technology and is coordinated by the Institute of Marine Biology, Biotechnology and Aquaculture of the Hellenic Centre for Marine Research, in conjunction with 47 associated partner institutions.

To know more about how Greece contributes to LifeWatch ERIC, please visit our dedicated webpage.

Italy

The Italian National Distributed Centre is led and managed by the Italian National Research Council (CNR) and is coordinated by a Joint Research Unit, currently comprising 35 members. Moreover, Italy hosts one of the LifeWatch ERIC Common Facilities, the Service Centre.

To know more about how Italy contributes to LifeWatch ERIC, please visit our dedicated webpage.

Netherlands

The Dutch National Distributed Centre is hosted by the Faculty of Science of the University of Amsterdam. Moreover, The Netherlands hosts one of the LifeWatch ERIC Common Facilities, the Virtual Laboratory and Innovation Centre.

To know more about how The Netherlands contributes to LifeWatch ERIC, please visit our dedicated webpage.

Portugal

The Portuguese National Distributed Centre is managed by PORBIOTA, the Portuguese e-Infrastructure for Information and Research on Biodiversity. Led by BIOPOLIS/CIBIO-InBIO – Research Centre in Biodiversity and Genetic Resources, PORBIOTA connects the principal Portuguese research institutions working in biodiversity.

To know more about how Portugal contributes to LifeWatch ERIC, please visit our dedicated webpage.

Slovenia

The Slovenian National Distributed Centre is led by the Research Centre of the Slovenian Academy of Sciences and Arts (ZRC SAZU). It focuses on the development of technological solutions in the field of biodiversity and socio-ecosystem research.

To know more about how Slovenia contributes to LifeWatch ERIC, please visit our dedicated webpage.

Spain

The Spanish National Distributed Centre is supported by the Ministry of Science, Innovation and Universities, the Regional Government of Andalusia and the Guadalquivir River Basin Authority (Ministry for Ecological Transition-MITECO). Moreover, Spain is the hosting Member State of LifeWatch ERIC, the location of its Statutory Seat & ICT e-Infrastructure Technical Office (LifeWatch ERIC Common Facilities). 

To know more about how Spain contributes to LifeWatch ERIC, please visit our dedicated webpage.

Bulgaria

The Bulgarian National Distributed Centre is represented by the  Agricultural University-Plovdiv.

To know more about how Bulgaria contributes to LifeWatch ERIC, please visit our dedicated webpage.

Mapping user requirements

  • End of January 2025 – Catalogue of services already available in LifeWatch ERIC or research lines addressing ecological responses to climate change;
  • February 2025 (TBD) – Online working table on setting priorities, timeline and milestones for the mapping service and model requirements by scientists and science stakeholders.

Implementing services

  • End of January 2025 – Internal distribution of a questionnaire on the most used/relevant model resources in the WG member research activity;
  • February 2025 (TBD) – Online working table on setting priorities, timeline and milestones for the mapping service and model requirements by scientists and science stakeholders.

Organising WG workshops and conferences

  • End of January 2025 – Setting priority research lines and contributions to the BEeS 2025 LifeWatch Conference for the session on the “Ecological responses to climate change”;
  • March/April 2025 (TBD) – Workshop ‘Ecological modelling and eco-informatics to address functional responses of biodiversity and ecosystems to climate change’ co-organised with the University of Salento;
  • 30 June – 3 July 2025 – Participation to LifeWatch 2025 BEeS Conference on “Addressing the Triple Planetary Crisis”.

Meetings, Webinars, International Conferences & Networking (2025/2026)

  • Organising and participating at discussions on emerging technologies in biodiversity monitoring;
  • Organising webinars on machine learning, eDNA analysis, and automated data collection;
  • Fostering collaboration between researchers, technologists, and decision-makers.

Collaborative Research & Case Studies (2025/2026)

  • Conducting pilot projects to test new monitoring methods;
  • Publishing scientific and popular science papers and reports on advancements in biodiversity assessment.

Data Standardisation & FAIR Principles Implementation (2025/2026)

  • Developing best practices for data curation and sharing;
  • Ensuring that biodiversity data aligns with FAIR (Findable, Accessible, Interoperable, Reusable) standards.

Development of VREs for Ecosystem Simulation (2026)

  • Creating virtual models of ecosystems to predict environmental changes;
  • Enhancing conservation strategies through AI-driven simulations.

Mapping Requirements and Gap Analysis

  • End of December 2025 – Catalogue of services already available in LifeWatch ERIC or research lines Ecosystem services mapping.

Methodological Alignment and Innovation

  • End of January 2026 – Online working table on mapping standards, classification systems, and indicators across members;
  • End of January 2026 – Catalogue of advanced techniques (e.g., remote sensing, GIS modelling, and machine learning) for scalable, habitat-based ecosystem service mapping;
  • End December 2026 – Methodological framework to support methodological innovation through joint development and testing of mapping approaches, especially linking ecosystem service supply and demand.

Knowledge Exchange and Capacity Building

  • End of December 2025 – Create a shared repository of guidance documents, tools, templates, and data resources accessible to WG members and broader communities.

Policy Relevance and Uptake

  • End of May 2026 – Policy-brief to demonstrate the application of habitat-based mapping in supporting EU strategies (e.g., Biodiversity Strategy, Nature Restoration Law).

Cross-Disciplinary Lexicon

  • End of April 2026 – Lexicon of standardised terminology
  • End of May 2026 – Publication submitted

Environmental Data Mapping

  • End of May 2026 – Core environmental data mapped as a list
  • End of June 2026 – Cross-comparison with existing LifeWatch catalogue done

Integrated Data-to-Model Workflows

  • End of December 2026 – At least 5 workflows for data-model integration developed and published
  • End of February 2027 – At least 5 workflows for policy-relevant outputs developed and published
Belgium

The Belgian National Distributed Centre makes varied and complementary in-kind contributions to LifeWatch ERIC. These are implemented in the form of long-lasting projects by various research centres and universities distributed throughout the country and supported by each respective political authority.

To know more about how Belgium contributes to LifeWatch ERIC, please visit our dedicated webpage.