The first day will be dedicated to the marine domain, with sessions focusing on the long-term monitoring of hard-bottom marine communities and marine/transitional NIS occurrence and trophic niches dynamics. The second day will be devoted to the terrestrial and freshwater domains, with a particular focus on the detection and monitoring of invaders through remote sensing and eDNA metabarcoding, and habitat vulnerability to NIS and impact on biotopes. Several round tables will enrich the workshop programme, covering hot topics within the biodiversity and ecosystem research community. As a follow-up to the workshop, a hands-on training session on the use of the five workflows and Virtual Research Environments (VRE) will be held on 4 June, subject to sufficient interest.